Point-of-care | Quantitative Vitamin D test
The VHC Quantitative Vitamin D Test allow healthcare professionals to measure “point of care” vitamin D in few minutes.
The test is an immunochromatography-based one step in vitro test. It is designed for the quantitative determination of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human finger-prick blood. This assay provides a preliminary diagnostic test result and can be used for screening of Vitamin D deficiency.
VHC Vitamin-D Quantitative Vitamin D Test utilizes the principle of immunochromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs a very “Exclusive” pair of anti-25-OH Vitamin D Monoclonal Antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity.
The liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays or other quantitative immunoassays is recommended to further confirm the diagnostic test results.
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Specimen collection and preparation
Pain free puncture with safety lancet
Adding light pressure a drop of blood emerge
The drop of blood is collected with the UniSampler™
5 drops of buffer liquid is added to the UniSampler™
The mix of blood and buffer is placed on the test window (S)
The total sample liquid should be 3-4 drops
VHC Reader adaptor is placed on top of the test
The VHC Reader is placed on the adaptor and the program is initiated.
The sensitivity of VHC Vitamin-D Quantitative Vitamin D Test is 3ng/ml (7.5nmol/l). The sensitivity was determined by calculating the mean plus 3.3 times of standard deviation of twenty Vitamin D-free serum tests.
30 Vitamin D free serum samples were tested and all showed negative results; suggesting 100% specificity. No interference and cross reactivity was observed with Bilirubin, Triglycerides, Cholesterol, Vitamin B12 and Vitamin C.
|Sample||No of replicates||Mean ng/ml||Standard deviation||Coeficient Variation (CV)|
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